| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Moorella thermoacetica | TGCCCTGTTCCTGCTCTTC | ATCAGCAAGACCAGGCCTG | 59.62 | 59.70 | 216 | 123 |
100.00%
|
100.00%
|
| Primer 2 | Moorella thermoacetica | GCTCAAACCACTTGCCGAC | TTGCCAGGATGTCGCTGT | 59.72 | 59.25 | 272 | 116 |
100.00%
|
100.00%
|
| Primer 3 | Moorella thermoacetica | TGCTCAAACCACTTGCCG | TTGCCAGGATGTCGCTGT | 58.57 | 59.25 | 273 | 110 |
100.00%
|
100.00%
|
| Primer 4 | Moorella thermoacetica | TTGAAGGCAATCGCGAGC | TACCTCGATGCGTTCCAGC | 58.82 | 59.86 | 161 | 109 |
100.00%
|
100.00%
|
| Primer 5 | Moorella thermoacetica | GTTGAAGGCAATCGCGAGC | TACCTCGATGCGTTCCAGC | 60.23 | 59.86 | 162 | 109 |
100.00%
|
100.00%
|
| Primer 6 | Moorella thermoacetica | ACGGGAAAAGGTGCAGCA | TGCTGATAGCCACGGCAT | 59.81 | 59.09 | 191 | 105 |
100.00%
|
100.00%
|
| Primer 7 | Moorella thermoacetica | AAAGGATTGGCGTCGGCA | ACGGACACTGATGACGGTC | 59.97 | 59.41 | 176 | 105 |
100.00%
|
100.00%
|
| Primer 8 | Moorella thermoacetica | TCCAGCGCACCGTCAAAA | AGCCAGCGCACATTGACA | 60.20 | 60.28 | 163 | 104 |
100.00%
|
100.00%
|
| Primer 9 | Moorella thermoacetica | AGGTAGACCTGCACCTGGT | TGGCAATCCACCGCTGAA | 59.84 | 59.57 | 151 | 103 |
100.00%
|
100.00%
|
| Primer 10 | Moorella thermoacetica | TGATGGATGTCAGCCTGCC | TGGCAATCCACCGCTGAA | 59.77 | 59.57 | 271 | 103 |
100.00%
|
100.00%
|